The only incident like this I can remember is Firefly:

Simon: “Are you Alliance?”

Jubal: “Am I a lion? …I don’t really think of myself as one. Though I do have a mighty roar!”

Simon: “…I said, are you Alliance”…

Jubal: “Oh. I thought you said…” Points gun more at Simon “Where’s your sister?”

I like it because it doesn’t really drive the plot or anything, it’s just the first time we see Jubal Early misstep, and seem awkward.